Pierce EZ-Link ™ Biotinylation Reagents make it easy to label any molecule (antibodies, peptides or oligonucleotides) with biotin to create a number of assay formats. Avidin’s affinity for biotin is so strong (Ka = 10-15 M-1) that it can survive boiling, pH extremes and detergents. Chaotropic agents such as 8M Guanidine•HCl or 6M Urea are the only agents that can dissociate this non-covalent bond.
Pierce can help you choose the right biotinylation reagent for your experimental applications. Whether you are performing Enzyme-Linked Immunosorbent Assay (ELISA), Western Blotting, Immunohistochemistry, Cell Surface Modification, Affinity Separations, Northern or Southern Blotting, Fluorescent Activated Cell Sorting, or Immunoprecipitation, the Avidin-Biotin complex can be used. Y not make it E-Z?