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Immunostimulants, Vaccines, and Environmental Stressors in Aquaculture: NBT Assays to show Neutrophil Activity by these Immunomodulator
Immunostimulants, Vaccines, and Environmental Stressors in Aquaculture: NBT Assays to show Neutrophil Activity by these
Immunomodulators Douglas P. Anderson Salmon Bay Biologics 2716 Elliott Ave. #900 Seattle, Washington 98121 USA
Abstract
While neutrophil activity assays have been used in human and veterinary medicine for diagnosis of blood neutrophil abnormalities, they are rarely used because of difficulties of handling nitro blue tetrazolium (NBT) solutions, drawing and preparation of blood samples and subjectivities in visual readings. We have developed modifications for a simplified NBT assay that can be used in the diagnosis and tracing the effectiveness of immunostimulants, vaccines and environmental stressors of fish. While training and practice for biologists and technicians is needed, the NBT assays can yield good results when applied to fish.
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