INFLUENCE OF DIMERIZED LYSOZYME (KLP-602) ON THE IMMUNE RESPONSES INDUCED BY FUROGEN VACCINE IN RAINBOW TROUT (ONCORHYNCHUS MYKISS)Andrzej K. Siwicki 1,3, Peter Klein2, Krzysztof Kazuń3,
Marc Morand4, Edward Głąbski3, Sylwia Trapkowska1
1Department of Microbiology and Clinical Immunology, University of Warmia and
Mazury in Olsztyn, Poland
2Nika Health Products Ltd, NJ, USA
3Department of Fish Pathology and Immunology, Inland Fisheries Institute in Olsztyn, Poland
4Laboratoire Veterinaire Departemental, Conseil General du Jura, France
Copyright © Wydawnictwo Akademii Rolniczej we Wroclawiu, ISSN 1505-0297
Electronic Journal of Polish Agricultural Universities
ABSTRACT
In the present study the possible stimulation of nonspecific and specific immune response after immunization induced by immersion Furogen (Aqua Health Ltd) vaccine were analysed in rainbow trout (Oncorhynchus mykiss). The modulation was attempted using a natural immunostimulant, dimerized lysozyme (KLP-602). Five groups of rainbow trout were given intraperitoneal injections of KLP-602 two days before vaccine, with vaccine, and two days after vaccine applicated by immersion. The results of cellular study showed that KLP-602 applicated before or with Furogen vaccine statistically significant (P<0.05) increased the total immunoglobulin secreting cells (ISC) and specific antibody secreting cells (ASC) levels, compared to the KLP-free (only vaccinated) group of fish. Analysis of the results shows no significant differences between group of fish where KLP-602 was applicated after vaccination and KLP-free (only vaccinated) group of fish. Analysis the results of humoral study shows that KLP-602 applicated before or with vaccine significantly increased (P<0.05) the total immunoglobulin levels and specific antibody levels in serum, compared to the KLP-free (only vaccinated) group of fish. Also results shows no significant differences between group of fish where KLP-602 was applicated after vaccination and KLP-free (only vaccinated) group of fish. The higher responses after the application of KLP-602 before or with vaccine were observed.
Key words: Lysozyme dimer, fish, Furogen vaccine, ASC, specific immune response
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